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KMID : 0644120160290020066
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Journal of Oriental Gynecology
2016 Volume.29 No. 2 p.66 ~ p.82
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Effects of Kanghwalsokdan-tang Gamibang Water Extract on Osteoclast Differentiation and Osteoblast Proliferation
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Jung Eun-Hye
Yoo Dong-Youl
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Abstract
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Objectives: This study was conducted to evaluate the effect of Kanghwalsokdantang Gamibang water extract (KSG) on osteoporosis.
Methods: RANKL-stimulated RAW 264.7 was used to evaluate inhibitory effect of KSG osteoclast differentiation and gene expression. We counted TRAP (+) multinucleated cells and measured TRAP activity and mRNA expressions of osteoclastogenesis-related genes (NFATc1, MITF, JNK1, cathepsin K, MMP-9) to figure out the effect of KSG on osteoclast. Osteoblastogenesis was also determined in rat calvarial cell. Alkaline phosphatase (ALP) activity, bone matrix protein and collagen synthesis were measured by using murine calvarial cell.
Results: KSG inhibited the differentiation of osteoclast precursor cell and expression of genes related osteoclastogenesis like NAFTc1, MITF, c-fos, JNK1, Cathepsin K, MMP-9 and TRAP. KSG increased cell division and function of osteoblast separated from the skull of a rat and ALP synthesis, biosynthesis of bone matrix protein and collagen.
Conclusions: Reviewing these results, KSG has efficacy on osteoclast inhibition and osteoblast activation. After further study, KSG will be able to apply for osteoporosis treatment and prevention.
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KEYWORD
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Kanghwalsokdan-tang Gamibang, osteoporosis, osteoclastogenesis, osteoblastogenesis, rat calvarial cell, osteoclast, osteoblast
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